Human Id-1 and Twist-1 promoter constructs
Bioinformatic analysis of human Id-1 and Twist-1
Design primers for human Id-1 and Twist-1 upstream regions
Grow and isolate sufficient quantity luciferase reporter vector
Isolate genomic DNA from fhMSC-SK-hTERT cells
Clone Id-1 and Twist-1 upstream regions via PCR
Ligate promoters into vectors
Transform fhMSC-SK-hTERT cells
oMake Id-1 and Twist-1 promoter/reporter constructs
oTo study transcriptional regulation of Twist-1 and Id-1 in differentiating MSCs

Perform luciferase assays
Grow cells and differentiate